Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

Rapid cell-free forward engineering of novel genetic ring oscillators

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

Cancers, Free Full-Text

Five-Year Outcomes for Refractory B-Cell Lymphomas with CAR T-Cell Therapy

Cells, Free Full-Text

Rapid cell-free characterization of multi-subunit CRISPR effectors and transposons - ScienceDirect

IJMS, Free Full-Text

Cell-free synthesis of human interferon. - Abstract - Europe PMC

Anode‐Free Full Cells: A Pathway to High‐Energy Density Lithium‐Metal Batteries - Nanda - 2021 - Advanced Energy Materials - Wiley Online Library

Cells, Free Full-Text