Transgenic Mice Expressing Green Fluorescent Protein under the
Por um escritor misterioso
Last updated 13 junho 2024
The melanocortin-4 receptor (MC4-R) is an important regulator of energy homeostasis, and evidence suggests that MC4-R-expressing neurons are downstream targets of leptin action. MC4-Rs are broadly expressed in the CNS, and the distribution of MC4-R mRNA has been analyzed most extensively in the rat. However, relatively little is known concerning chemical profiles of MC4-R-expressing neurons. The extent to which central melanocortins act presynaptically or postsynaptically on MC4-Rs is also unknown. To address these issues, we have generated a transgenic mouse line expressing green fluorescent protein (GFP) under the control of the MC4-R promoter, using a modified bacterial artificial chromosome. We have confirmed that the CNS distribution of GFP-producing cells is identical to that of MC4-R mRNA in wild-type mice and that nearly all GFP-producing cells coexpress MC4-R mRNA. For example, cells coexpressing GFP and MC4-R mRNA were distributed in the paraventricular hypothalamic nucleus (PVH) and the dorsal motor nucleus of the vagus (DMV). MC4-R promotor-driven GFP expression was found in PVH cells producing thyrotropin-releasing hormone and in cholinergic DMV cells. Finally, we have observed that a synthetic MC3/4-R agonist, MT-II, depolarizes some GFP-expressing cells, suggesting that MC4-Rs function postsynaptically in some instances and may function presynaptically in others. These studies extend our knowledge of the distribution and function of the MC4-R. The transgenic mouse line should be useful for future studies on the role of melanocortin signaling in regulating feeding behavior and autonomic homeostasis.
GFP-tagging in Fluorescence Microscopy
Figure 1. Neuromedin B and Gastrin-Releasing Peptide Excite
Germline mutagenesis mediated by Sleeping Beautytransposon system
Reagents and models for detecting endogenous GLP1R and GIPR
Fluorescent Proteins Expressed in Mouse Transgenic Lines Mark
Fluorescence Microscopy
Introducing AntiCancer
Choosing Fluorescent Reagents for Every Live Cell Application
Green Fluorescent Protein in Transgenic Mice
Destabilized green fluorescent protein detects rapid removal of
Green Fluorescent Protein in Transgenic Mice
Figures and data in Scn1a-GFP transgenic mouse revealed Nav1.1
Recomendado para você
-
scpcb/NPCs.bb at master · Regalis11/scpcb · GitHub13 junho 2024
-
SCP 7143 J THE KNOB [SFW?]13 junho 2024
-
Gooey Earrings - Drawception13 junho 2024
-
Banging on the Door, SCP Foundation13 junho 2024
-
Picture memes vksFDjFX6 by I_eat_ass____2017 - iFunny Brazil13 junho 2024
-
SCP 6034 - The War With Another Dimension13 junho 2024
-
I can't believe this happened 😂 #screammovie #ghostface #scream6 #scr13 junho 2024
-
PDF) Tractographical model of the cortico-basal ganglia and corticothalamic connections: Improving Our Understanding of Deep Brain Stimulation13 junho 2024
-
Journal of Comparative Neurology, Systems Neuroscience Journal13 junho 2024
-
US20190218569A1 - Isolated polynucleotides and polypeptides and methods of using same for increasing plant yield, biomass, growth rate, vigor, oil content, abiotic stress tolerance of plants and nitrogen use efficiency - Google Patents13 junho 2024
você pode gostar
-
Reviews: Kamisama Kiss - IMDb13 junho 2024
-
FIFA World Cup 2018 Schedule: Start Times, Odds For Every Game, Predictions, Groups, Bracket, More13 junho 2024
-
Meme of dora the explorer with a chad face13 junho 2024
-
Jogos de Aventura: Jogar grátis online no Reludi13 junho 2024
-
jogos de moto realista para13 junho 2024
-
Película Adesivo Cartão Pokemon Segunda Geração Miltank Raikou13 junho 2024
-
Ghost of Tsushima Director's Cut Review: Adds Great New Features13 junho 2024
-
Hellblade II Teaser Impressionante Face Animation - TGS13 junho 2024
-
How Much Money Do You Have To Invest To Make Money on a Meme Crypto?13 junho 2024
-
Tênis Converse All Star Cano Baixo Preto Unissex 13604413 junho 2024